Hieff clone enzyme
Web27 de fev. de 2024 · Metabolic engineering usually focuses on manipulating enzyme(s) within a single pathway. ... using Hieff Clone Plus Multi One Step Cloning Kit, resulting …
Hieff clone enzyme
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WebDigest your DNA: Set up restriction digests for your donor and recipient plasmids. Because you lose some DNA during the gel purification step, it is important to digest plenty of starting material. We recommend 1.5-2μg of … Web19 de jan. de 2024 · Then, AQP1 gene fragment (GenBank, NM_198098.3) was cloned into pGMLV-hTERT-MCS-EGFP using a Hieff Clone™ One Step Pcr Cloning Kit to generate pGMLV-hTERT-AQP1-EGFP plasmid. pGMLV-CMV-AQP1 ... All of the constructed plasmids were identified by restriction enzyme digestion analysis and DNA sequencing. …
WebCompared with the traditional enzyme ligation technology, the seamless cloning kit is simple and fast to operate, and is not limited by the restriction of the enzyme cleavage site. It can splicing multiple fragments at one time, which greatly improves the cloning efficiency. This Hieff Clone™ universal one step cloning kit is available for 1 ... WebHieff Clone™ Zero TOPO-Blunt Cloning Kit, one topo cloning kit, is developed for rapid cloning of blunt-end PCR products and rapid sequencing of PCR products after …
WebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 … Web23 de dez. de 2024 · To complement the bwd1 mutant, the coding region of AAE9 was induced into the pFGC-eYFP vector using the Hieff Clone Plus One Step Cloning Kit (Yeasen Biotechnology (Shanghai) Co. Ltd, Shanghai, China) driven by the 35S promoter to generate the 35S::AAE9-eYFP construct.
Web1 de nov. de 2024 · Enzymes immobilized on solid supports have been certified to improve biocatalytic reusability. Hence, the immobilization of the polymers on solid ... amplified products were inserted into the recombinant plasmids pET22b-Linker1-SpyTag and pET22b-Linker2-SpyCatcher by Hieff Clone Plus One Step Cloning Kit (Yeasen Biotech Co., Ltd ...
● No need to consider the restriction site: Insert fragment restriction site-free, compatible with any vector, sticky or blunt ends. ● Easy-to-use: Insert a 20-bp vector-end homolog at the 5' end of the inserted fragment's PCR primer. ● Fast and efficient: Recombination takes 5 minutes at 50 °C. Clones can … Ver mais ① Preparation of linearized vector: preparation of linearized vector by enzyme digestion or PCR. ② Preparation of insert fragment: At the 5' end of the upstream and downstream primers of the target DNA segment, add 15 … Ver mais Q: What is the principle of Hieff Clone™ cloning? A: Using the principle of terminal homologous recombination. Any linearized vector and DNA … Ver mais in 8 hours what time will it beWeb2× Plus PCR Master Mix (With Dye) is a ready-to-use 2x premixed solution containing Hieff Canace™ Plus high-fidelity DNA polymerase, dNTPs, and an optimized buffer. This PCR … in 800aWeb产品描述 . Hieff Clone ® Universal One Step Cloning Kit 是新一代的同源重组克隆试剂盒，精心优化的 2×Hieff Clone ® Universal Enzyme Premix 预混了重组酶和重组反应所需缓冲液，并添加了独特的重组增强因子，可 … ina garten recipe for chuck roastWebGolden Gate Assembly is another method of seamless cloning that exploits the ability of Type IIS restriction enzymes (such as BsaI-HF ® v2) to cleave DNA outside of the recognition sequence. The inserts and … in 8 moyockWeb655.00. 产品描述. Hieff Clone ® Universal One Step Cloning Kit 是新一代的同源重组克隆试剂盒，精心优化的 2×Hieff Clone ® Universal Enzyme Premix 预混了重组酶和重组反 … in 8/16 time how many beats in one measureWebThis Hieff Clone™ universal one step cloning kit is available for 1-6 fragment cloning. Products in stock in the United States can be delivered within 2 days. +1 2404726069 … ina garten recipe for brown butter cornbreadWebHIF-1 alpha cDNA clone Background. All these HIF-1 alpha cDNA clone are full sequence confirmed. There are 15 HIF-1 alpha expression cDNA clones with various fusion tags, … in 8051 timer 1 run can be controlled by